The Immortal Life of Henrietta Lacks

by Rebecca Skloot

Henrietta died in 1951 from a vicious case of cervical cancer, he told us. But before she died, a surgeon took samples of her tumor and put them in a petri dish. Scientists had been trying to keep human cells alive in culture for decades, but they all eventually died. Henrietta’s were different: they reproduced an entire generation every twenty-four hours, and they never stopped. They became the first immortal human cells ever grown in a laboratory. “Henrietta’s cells have now been living outside her body far longer than they ever lived inside it,” Defler said. If we went to almost any cell culture lab in the world and opened its freezers, he told us, we’d probably find millions—if not billions—of Henrietta’s cells in small vials on ice. Her cells were part of research into the genes that cause cancer and those that suppress it; they helped develop drugs for treating herpes, leukemia, influenza, hemophilia, and Parkinson’s disease; and they’ve been used to study lactose digestion, sexually transmitted diseases, appendicitis, human longevity, mosquito mating, and the negative cellular effects of working in sewers. Their chromosomes and proteins have been studied with such detail and precision that scientists know their every quirk. Like guinea pigs and mice, Henrietta’s cells have become the standard laboratory workhorse.

When I go to the doctor for my checkups I always say my mother was HeLa. They get all excited, tell me stuff like how her cells helped make my blood pressure medicines and antidepression pills and how all this important stuff in science happen cause of her. But they don’t never explain more than just sayin, Yeah, your mother was on the moon, she been in nuclear bombs and made that polio vaccine. I really don’t know how she did all that, but I guess I’m glad she did, cause that mean she helpin lots of people. I think she would like that.

All cancers originate from a single cell gone wrong and are categorized based on the type of cell they start from. Most cervical cancers are carcinomas, which grow from the epithelial cells that cover the cervix and protect its surface. By chance, when Henrietta showed up at Hopkins complaining of abnormal bleeding, Jones and his boss, Richard Wesley TeLinde, were involved in a heated nationwide debate over what qualified as cervical cancer, and how best to treat it.

working to grow malignant cells outside the body, hoping to use them to find cancer’s cause and cure. But most cells died quickly, and the few that survived hardly grew at all. The Geys were determined to grow the first immortal human cells: a continuously dividing line of cells all descended from one original sample, cells that would constantly replenish themselves and never die. Eight years earlier—in 1943—a group of researchers at the National Institutes of Health had proven such a thing was possible using mouse cells. The Geys wanted to grow the human equivalent—they didn’t care what kind of tissue they used, as long as it came from a person. Gey took any cells he could get his hands on—he called himself “the world’s most famous vulture, feeding on human specimens almost constantly.” So when TeLinde offered him a supply of cervical cancer tissue in exchange for trying to grow some cells, Gey didn’t hesitate. And TeLinde began collecting samples from any woman who happened to walk into Hopkins with cervical cancer. Including Henrietta.

But first—though no one had told Henrietta that TeLinde was collecting samples or asked if she wanted to be a donor—Wharton picked up a sharp knife and shaved two dime-sized pieces of tissue from Henrietta’s cervix: one from her tumor, and one from the healthy cervical tissue nearby. Then he placed the samples in a glass dish.

But Carrel wasn’t interested in immortality for the masses. He was a eugenicist: organ transplantation and life extension were ways to preserve what he saw as the superior white race, which he believed was being polluted by less intelligent and inferior stock, namely the poor, uneducated, and nonwhite. He dreamed of never-ending life for those he deemed worthy, and death or forced sterilization for everyone else. He’d later praise Hitler for the “energetic measures” he took in that direction.

Reader’s Digest ran articles by Carrel advising women that a “husband should not be induced by an oversexed wife to perform a sexual act,” since sex drained the mind. In his best-selling book, Man, the Unknown, he proposed fixing what he believed was “an error” in the U.S. Constitution that promised equality for all people. “The feebleminded and the man of genius should not be equal before the law,” he wrote. “The stupid, the unintelligent, those who are dispersed, incapable of attention, of effort, have no right to a higher education.”

In early June, Henrietta told her doctors several times that she thought the cancer was spreading, that she could feel it moving through her, but they found nothing wrong with her. “The patient states that she feels fairly well,” one doctor wrote in her chart, “however she continues to complain of some vague lower abdominal discomfort. … No evidence of recurrence. Return in one month.”

Sadie would later describe Henrietta’s decline like this: “Hennie didn’t fade away, you know, her looks, her body, it didn’t just fade. Like some peoples be sick in the bed with cancer and they look so bad. But she didn’t. The only thing you could tell was in her eyes. Her eyes were tellin you that she wasn’t gonna be alive no more.”

“I’ll never forget it,” Aurelian said. “George told me he leaned over Henrietta’s bed and said, ‘Your cells will make you immortal.’ He told Henrietta her cells would help save the lives of countless people, and she smiled. She told him she was glad her pain would come to some good for someone.”

What rolled in front of me on that television screen was a one-hour BBC documentary about Henrietta and the HeLa cells, called The Way of All Flesh, which I’d been trying to get a copy of for months. It opened to sweet music and a young black woman who wasn’t Henrietta, dancing in front of the camera. A British man began narrating, his voice melodramatic, like he was telling a ghost story that just might be true.

Black scientists and technicians, many of them women, used cells from a black woman to help save the lives of millions of Americans, most of them white. And they did so on the same campus—and at the very same time—that state officials were conducting

the infamous Tuskegee syphilis studies.

Viruses reproduce by injecting bits of their genetic material into a living cell, essentially reprogramming the cell so it reproduces the virus instead of itself. When it came to growing viruses—as with many other things—the fact that HeLa was malignant just made it more useful. HeLa cells grew much faster than normal cells, and therefore produced results faster. HeLa was a workhorse: it was hardy, it was inexpensive, and it was everywhere.

Researchers had long believed that human cells contained forty-eight chromosomes, the threads of DNA inside cells that contain all of our genetic information. But chromosomes clumped together, making it impossible to get an accurate count. Then, in 1953, a geneticist in Texas accidentally mixed the wrong liquid with HeLa and a few other cells, and it turned out to be a fortunate mistake. The chromosomes inside the cells swelled and spread out, and for the first time, scientists could see each of them clearly. That accidental discovery was the first of several developments

that would allow two researchers from Spain and Sweden to discover that normal human cells have forty-six chromosomes.

Reader recruited the top minds in the field to tell him what products they needed most and show him how to make them. One of the scientists who consulted for Reader was Leonard Hayflick, arguably the most famous early cell culturist left in the field today. When I talked with him he said, “Microbiological Associates and Sam Reader were an absolute revolution in the field, and I’m not one to use the word revolution lightly.”

The possibilities seemed endless. At one point, a health-education director at the Young Women’s Christian Association heard about tissue culture and wrote a letter to a group of researchers saying she hoped they’d be able to use it to help the YWCA’s older women. “They complain that the skin and tissues of the face and neck inevitably show the wear and tear of years,” she wrote. “My thought was that if you know how to keep tissue alive there must be some way of equalizing the reserve supply to the area of the throat and face.”

Henrietta’s cells couldn’t help bring youth to women’s necks, but cosmetic and pharmaceutical companies throughout the United States and Europe began using them instead of laboratory animals to test whether new products and drugs caused cellular damage. Scientists cut HeLa cells in half to show that cells could live on after their nuclei had been removed, and used them to develop methods for injecting substances into cells without destroying them. They used HeLa to test the effects of steroids, chemotherapy drugs, hormones, vitamins, and environmental stress; they infected them with tuberculosis, salmonella, and the bacterium that causes vaginitis.

With regard to your … disapproval for a wide exploration of the HeLa strain, I don’t see how you can hope to inhibit progress in this direction since you released the strain so widely that it now can be purchased commercially. This is a little bit like requesting people not to work on the golden hamster! … I realize that it is the goodness of your heart that made available the HeLa cell and therefore why you now find that everybody wants to get into the act. Pomerat suggested that Gey should have finished his own HeLa research before “releasing [HeLa] to the general public since once released it becomes general scientific property.” But Gey hadn’t done that. And as soon as HeLa became “general scientific property,” people started wondering about the woman behind the cells.

Soon after, Deborah asked Bobbette what pregnant was. Bobbette told her, then grabbed Deborah’s shoulders again and told her to listen good. “I know your mother and father and all the cousins all mingled together in their own way, but don’t you ever do it, Dale. Cousins are not supposed to be havin sex with each other. That’s uncalled for.”

Bobbette had told Deborah that maybe she and her siblings had hearing problems because their parents were first cousins. Deborah knew other cousins had children who were dwarves, or whose minds never developed. She wondered if that had something to do with what happened to Elsie.

In February 1954, Southam loaded a syringe with saline solution mixed with HeLa. He slid the needle into the forearm of a woman who’d recently been hospitalized for leukemia, then pushed the plunger, injecting about five million of Henrietta’s cells into her arm. Using a second needle, Southam tattooed a tiny speck of India ink next to the small bump that formed at the HeLa injection site. That way, he’d know where to look when he reexamined the woman days, weeks, and months later, to see if Henrietta’s cancer was growing on her arm. He repeated this process with about a dozen other cancer patients. He told them he was testing their immune systems; he said nothing about injecting them with someone else’s malignant cells.

Within hours, the patients’ forearms grew red and swollen. Five to ten days later, hard nodules began growing at the injection sites. Southam removed some of the nodules to verify that they were cancerous, but he left several to see if the patients’ immune systems would reject them or the cancer would spread. Within two weeks, some of the nodules had grown to two centimeters—about the size of Henrietta’s tumor when she went in for her radium treatments.

Southam eventually removed most of the HeLa tumors, and those he didn’t remove vanished on their own in a few months. But in four patients, the nodules grew back. He removed them, but they returned again and again. In one patie...

Since those patients had all had cancer to beg...

wanted to see how healthy people reacted to the injections, for comparison’s sake. So, in May 1956, he placed an ad in the Ohio State Penitentiary newsletter: Physician seeks 25 volunteers for cancer research. A few days later ...

Southam gave multiple cancer cell injections to each prisoner, and unlike the terminally ill patients, those men fought off the cancer completely. And with each new injection, their bodies responded faster, which seemed to indicate that the cells were increasing the inmates’ immunity to cancer. When Southam reported his results, the press hailed them as a tremendous breakthrough that could someday lead to a cancer vaccine.

In the coming years, Southam injected HeLa and other living cancer cells into more than six hundred people for his research, about half of them cancer patients. He also began injecting them into every gynecologic surgery patient who came to Sloan-Kettering’s Memorial Hospital or its James Ewing Hospital. If he explained anything, he simply said he was testing them for cancer. And he believed he was: Since people with cancer seemed to reject the cells more slowly than healthy people did, Southam thought that by timing the rejection rate, he might be able to find undiagnosed cases of cancer.

In a statement he’d later repeat again and again during hearings about his research, Southam wrote, “It is, of course, inconsequential whether these are cancer cells or not, since they are foreign to the recipient and hence are rejected. The only drawback to the use of c...

dreaded word ‘cancer’ in connection with any clinical procedure on an ill person is potentially deleterious to that patient’s well-being, because it may suggest to him (rightly or wrongly) that his diagnosis is cancer or that his prognosis is poor. … To withhold such emotionally disturbing but medically nonpertinent details … is in the best tradition of responsible clinical practice.”

But Southam wasn’t their doctor, and he wasn’t withholding upsetting health information. The deception was for his benefit—he was withholding information because patients might have refused to participate in his study if they’d known what he was injecting. And Southam probably would have continued doing this for years had he not made an arrangement on July 5, 1963, with Emanuel Mandel, director of medicine at the Jewish Chronic Disease Hospital in Brooklyn, to use the hospital’s patients for his research.

The plan was that Mandel would have doctors on his staff inject twenty-two JCDH patients with cancer cells for Southam. But when he instructed his staff to give the injections without telling patients they contained cancer cells, three young Jewish doctors refused, saying they wouldn’t conduct research on patients without their consent. All three knew about the...

Sixteen years earlier, on August 20, 1947, a U.S.-led war tribunal in Nuremberg, Germany, had sentenced seven Nazi doctors to death by hanging. Their crime was conducting unthinkable research on Jews without consent—sewing siblings together...

func...

The tribunal set forth a ten-point code of ethics now known as the Nuremberg Code, which was to govern all human experimentation worldwide. The first line in that code says, “The voluntary consent of the human subject is absolutely essential.” The idea was revolutionary. The Hippocratic Oath, written in the fourth century BC, didn’t require patient consent. And though the American Medical Association ...

But the Nuremberg Code—like other codes that would come after it—wasn’t law. It was, essentially, a list of recommendations. It wasn’t routinely taught in medical schools, and many American researchers—including Southam—claimed not to know it existed. Those who did know about it often thought of it as “the Nazi ...

The term informed consent first appeared in court documents in 1957, in a civil court ruling on the case of a patient named Martin Salgo. He went under anesthesia for what he thought was a routine procedure and woke up permanently paralyzed from the waist down. The doctor hadn’t told him the procedure carried any risks at all. The judge ruled against the doctor, saying, “A physician violates his duty to his patient and subjects himself to liability if he withholds any facts which are necessary to form the basis of an intelligent consent by the patient to the proposed treatment.” He wrote that there needed to be “full disclosure of facts necessary to an informed consent.”

They said the Nuremberg Code didn’t seem to apply in the United States, and that there were no laws protecting research subjects. Science magazine called it “the hottest public debate on medical ethics since the Nuremberg trials,” and said, “The situation at present appears rather perilous for everyone.” A reporter from Science asked Southam why, if the injections were as safe as he swore they were, he didn’t inject himself. “Let’s face it,” Southam responded, “there are relatively few skilled cancer researchers, and it seemed stupid to take even the little risk.”

These patients then had a right to know … the contents of the syringe: and if this knowledge was to cause fear and anxiety or make them frightened, they had a right to be fearful and frightened and thus say NO to the experiment.”

In 1965 two British scientists, Henry Harris and John Watkins, took cell sex an important step further. They fused HeLa cells with mouse cells and created the first human-animal hybrids—cells that contained equal amounts of DNA from Henrietta and a mouse. By doing this, they helped make it possible to study what genes do, and how they work.

In addition to the HeLa-mouse hybrid, Harris fused HeLa with chicken cells that had lost their ability to reproduce. His hunch was that when those deactivated chicken cells fused with HeLa, something inside HeLa would essentially turn the chicken cell back on. He was right. He didn’t know how it worked yet, but his discovery showed that something in cells regulated genes. And if scientists could figure out how to turn disease genes off, they might be able to create a form of gene therapy.

Without knowing anything about Joe’s life or the abuse he experienced as a child, his lawyer said, “He feels it more necessary to protect himself than the average individual. And possibly, this sets him off, where it would not set off the average person.”

Few there had heard of Stanley Gartler, but that was about to change. Gartler leaned into the microphone and told the audience that, in the process of looking for new genetic markers for his research, he’d found that eighteen of the most commonly used cell cultures had one thing in common: they all contained a

rare genetic marker called glucose-6-phosphate dehydrogenase-A (G6PD-A), which was present almost exclusively in black Americans. And even among them it was fairly rare.

When Gey responded that HeLa cells had come from “a colored woman,” Gartler knew he’d found the source of the problem. “It seems to me the simplest explanation,” he told the audience, “is that they are all HeLa cell contaminants.” Scientists knew they had to keep their cultures free from bacterial and viral contamination, and they knew it was possible for cells to contaminate one another if they got mixed up in culture. But when it came to HeLa, they had no idea what they were up against. It turned out Henrietta’s cells could float through the air on dust particles. They could travel from one culture to the next on unwashed hands or used pipettes; they could ride from lab to lab on researchers’ coats and shoes, or through ventilation systems. And they were strong: if just one HeLa cell landed in a culture dish, it took over, consuming all the media and filling all the space.

Everyone in the audience knew what that meant. On top of saying they’d possibly wasted more than a decade and millions of research dollars, Gartler was also suggesting that spontaneous transformation—one of the most celebrated prospects for finding a cure for cancer—might not exist. Normal cells didn’t spontaneously become cancerous, he said; they were simply taken over by HeLa.

“A few years ago I voiced some suspicion about cell-line contamination,” Hsu said. “So I am happy about the paper by Dr. Gartler and am also sure he has made many people unhappy.”

Harvard’s Robert Chang—whose widely used Chang Liver Cell line was listed as a HeLa contaminant on Gartler’s chart—glared from his seat. Chang had used those cells to discover enzymes and genes specific to liver cells. If Gartler was right and the cells were actually from Henrietta’s cervix, Chang’s liver research using them was worthless.

Leonard Hayflick had an especially personal connection with his cell line, WISH, which Gartler had listed as contaminated: he’d grown it using cells from the amniotic sac in which his unborn daughter had once floated. He asked Gartler whether it was possible to find G6PD-A in samples from white people. “Caucasian subjects with G6PD-A have not been reported,” Gartler told him.