(1) the organism/microbial cell must be found in a diseased individual, not in a healthy individual; (2) the microbial cell must be isolated and cultured from the diseased individual; (3) the inoculation of a healthy individual with the cultured microbe must recapitulate the essential features of the disease; and (4) the microbe must be re-isolated from the inoculated individual and match the original microorganism.I
