In order to prepare for their long winter journey, trees undergo a process known as “hardening.” First the permeability of the cell walls increases drastically, allowing pure water to flow out while concentrating the sugars, proteins, and acids left behind. These chemicals act as a potent antifreeze, such that the cell can now dip well below freezing and the fluid inside of it will still persist in a syrupy liquid form. The spaces between the cells are now filled with an ultra-pure distillate of cell water, so pure that there are no stray atoms upon which an ice crystal could nucleate and
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