It was only decades later that Lister abandoned the carbolic spray when the German physician and microbiologist Robert Koch developed a technique for staining and growing bacteria in a Petri dish (named after his assistant Julius Petri). This enabled Koch to match particular microorganisms to specific diseases and advance the theory that bacteria exist as distinct species, each producing a unique clinical syndrome. Using his method, Koch showed that airborne pathogens were not the main culprit of wound infection, which meant that sterilizing the air was futile.

