Mushrooms are cloned by placing a live piece of shroom into the agar. This will cause the mushroom to grow into a culture that can later be used for inoculation. The first step is to choose a large shroom as it will be easier to handle and is more likely to be fully developed. For the best results, the mushroom should not be older than 2 days after harvest. Wipe down the mushroom with alcohol, removing any debris, substrate or contams from its surface. At this point, the mushroom is no longer safe to eat but is perfect for propagation.
