Throughout their occupation, the Japanese had used biological and chemical weapons. Lethal experiments were conducted on prisoners of war in a string of secret laboratories stretching from northern Manchuria to subtropical Guangdong. Victims were subjected to vivisection without anaesthesia after being infected with various germs. Others had their limbs amputated, their stomachs excised or parts of their organs surgically removed. Weapons, including flamethrowers and chemical agents, were tested on prisoners tied to stakes.