Molecular Cloning: A Laboratory Manual, Third Edition

by Joseph Sambrook, David Russell

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using todays biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

Genres: Science, Biology, Nonfiction, Textbooks

2344 pages, Paperback

First published December 1, 1989

Reviews

[Matthias Walters · Rating 5 out of 5]

Of course, didn’t read this cover to cover; however, having referenced these textbooks often when doing new experiments and now having read the 300-page appendix, I consider this to be the BEST resource on molecular biology techniques and materials. SUPER SET!!

[Aaron · Rating 5 out of 5]

like the bible, but with information i can use every day.

[widhi adrianna · Rating 5 out of 5]

This is IMPORTANT book for the scientists of molecular biolgy

[Shreyashree · Rating 4 out of 5]

Apt, To the point and in lucid writings, this book aims to teach easy to complex molecular biology techniques to the students. What is exceptionally appealing about this book is that it addresses critical problems that are bound to crop up in experiments and the resolutions of the same. A must read for all those keen towards research in molecular biology (specifically RNA biologists).

[Sofia · Rating 5 out of 5]

This is the closest thing a molecular biologist has to Holy Scripture. There isn't a single molbio lab in the world that doesn't worship at the altar of Saint Sambrook, patron of DNA, RNA and protein. Goodreads needs a new rating system for this, because five stars isn't enough.

[Ahmad · Rating 4 out of 5]

manual keteknikan asam nukleat yang pertama kali saya baca sewaktu intership di LBM Eijkman, ada 3 jilid gitu, tebal pula, sebuah "kitab suci"-nya biologi molekuler

[Ayu Saraswati · Rating 4 out of 5]

buku wajib zaman skripsi

[Adrianna · Rating 5 out of 5]

Important book for someone in molecular biology field