Methods in Cell Biology, Volume 29: Fluorescence Microscopy of Living Cells in Culture, Part A : Fluorescent Analogs, Labeling Cells, and Basic Microscopy
Parts A and B, companion volumes on fluorescence microscopy, are sourcebooks of methods for living cells. Part A comprehensively covers fluorescent analogs, procedures for fluorescent labeling of various cellular structures, and fluorescence microscopy. Part B describes instrumentation and quantitation analysis for imaging and spectroscopy.
Key Features * Convenient bench-top format Comprehensive methods inluded * Tracing proteins, peptides, hormones, and toxins * Labeling cell surfaces, mitochondria, endoplasmic reticulum, and endocytic compartments * Preparation of fluorescent probes and their incorporation into cells * Culturing cells under the microscope * Basic fluorescence microscopy and imaging techniques