RNA Processing: A Practical ApproachVolume II

by Stephen J. Higgins (editor), B. David Hames (Editor)

As post-transcriptional events become increasingly recognized as critical regulation sites of gene expression in eukaryotic cells, research interest in RNA processing has grown more and more intense. RNA A Practical Approach , available in two volumes, offers detailed guidance on
all major aspects of the subject. Step-by-step protocols from leading laboratories are presented for studying the termination, end-processing, capping, methylation, splicing, and editing of mRNA. Protocols designed for the study of mRNA stability and processing of rRNA and tRNA are included as
well, with full descriptions of the synthesis and purification of RNA substrate for in vitro work, the characterization of specific RNAs, and the isolation and analysis of ribonucleoprotein complexes. Taken together, the two volumes are invaluable laboratory companions to researchers working in
mRNA, rRNA, and tRNA expression. The contributors' lucid explanatory style and comprehensive coverage will be welcomed by both experienced researchers and students embarking on such studies for the first time.

280 pages, Paperback

First published March 24, 1994