Methods in Enzymology, Volume 195: Adenylyl Cyclase, G Proteins, and Guanylyl Cyclase

by Sidney P. Colowick (Editor), Roger A. Johnson (Editor), Melvin I. Simon (Editor)

This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases.
Key Features
* Adenylyl cyclase: determination of activity
* Preparation of materials useful in purification of components of hormonally responsive cyclase systems
* Purification guanine nucleotide-dependent regulatory proteins
* Purification and characterization of g proteins: labeling and quantitating of G proteins
* Reconstitution guanylyl cyclase: assay
* Preparation of materials used for study of the cyclase
* Purification
* Regulation

511 pages, Hardcover

First published February 4, 1991