Methods in Molecular Biology, Volume 99: Stress Response: Methods and Protocols

by Stephen M. Keyse (Editor)

In is now understood that the response of mammalian cells to a wide va riety of potentially toxic agents may be intimately linked with many h uman diseases, including rheumatoid arthritis, ischemia, fever, infect ion, and cancer. In Stress Response: Methods and Protocols, Stephen Ke yse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses. Written by leading researchers expert in the techniques they describe, these d etailed methods cover the detection and assay of stress-induced damage, the activation of a wide range of signal transduction pathways by ce llular stress, stress-induced gene expression, and stress protein func tion. To ensure experimental success, step-by-step guidance is provide d for each method, along with details of reagents, equipment, and othe r requirements. The methods include both well-established techniques a nd new technologies at the leading edge of research.

504 pages, Hardcover

First published May 31, 2000