RT-PCR is now a fundamental tool for biological and biomedical research and diagnostics. This unique book, devoted to the real-life applications of the polymerase chain reaction (PCR) methodology, takes a trouble-shooting approach. It is both timely and topical, reflecting the immense experience of the authors, regarded as pioneers in this field of research. Unlike many existing texts on the topic, it is up-to-date and pragmatic, and will be well received by research communities and industrial researchers alike.