After explaining the basic science behind the Polymerase Chain Reaction, this lab manual provides protocols for sample preparation, RT-PCR methods, detection of PCR products, analysis of differential expression, cloning, and mutagenesis. Appendices offer information on licensing, modification of oligonucleotides, hazards, and suppliers. The 31 chapters represent the work of scientists from around the country. Annotation (c) Book News, Inc., Portland, OR (booknews.com)