Fundamentals of Light Microscopy and Electronic Imaging

by Douglas B. Murphy

Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope–skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image. Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions. An essential resource for both, experienced and novice microscopists.

360 pages, Hardcover

First published October 29, 2001

Reviews

[Martina · Rating 5 out of 5]

Fundamentals of Light Microscopy and Electronic Imaging is a great book on LM. I really enjoyed this book for more than just the thorough explanations. You see, as a theoretician, I keep somewhat of a distance towards experimental methods... I know, it's not the most flattering thing to admit, but I do appreciate experimental methods as a whole. Unfortunately, with so many exciting and challenging methods (EPR, anyone?), I deemed light microscopy as not interesting enough and passe.

Boy, did this book change my mind! That's the main reason I gave this book five stars - because it has demystified LM to me, proving that I was wrong to have prejudice towards it. Just after going through the book I realize how much thought and cleverness and good ol' physics was necessary to invent techniques such as phase-contrast or differential-interference-contrast. This book has once again showed me just how amazing physics is.

I realize that my review is very subjective, but I would still encourage everyone to pick up this book. The explanations are good, despite the fact that there is little math, but Murphy compensates that with illustrations and graphs galore.