Introducing Proteomics: From Concepts to Sample Separation, Mass Spectrometry and Data Analysis

by Josip Lovric

Introducing Proteomics gives a concise and coherent overview of every aspect of current proteomics technology, which is a rapidly developing field that is having a major impact within the life and medical sciences. This student-friendly book, based on a successful course developed by the author, provides its readers with sufficient theoretical background to be able to plan, prepare, and analyze a proteomics study. The text covers the This contemporary text also includes numerous examples and explanations for why particular strategies are better than others for certain applications. In addition, Introducing Proteomics includes extensive references and a list of relevant proteomics information sources; essential for any student. This no-nonsense approach to the subject tells students exactly what they need to know, leaving out unnecessary information. The student companion site enhances learning and provides answers to the end of chapter problems. "I think this book will be a popular and valuable resource for students and newcomers to the field who would like to have an overview and initial understanding of what proteomics is about. The contents are well organized and address the major issues."
― Professor Walter Kolch , Director, Systems Biology Ireland & Conway Institute, University College Dublin Companion Website

304 pages, Paperback

First published March 11, 2010

Reviews

[Sarah · Rating 4 out of 5]

I got this book because I didn't know very much about proteomics aside from "do some 2D gels and some mass spec." This book is primarily an overview of modern technology, technique, and databases, which are discussed in chapters 1-4. The last chapter is a snapshot of some recent publications in proteomics, as well as future directions of the field.

Things I appreciated in this book include: no background on proteins (translation and modification); overview of strengths and weaknesses of various systems and methods; clear definitions of MALDI, ESI, MS/MS, and ion traps; extensive list of protein databases, with a summary/review for each.

Things I did not appreciate include: somewhat awkward organization (I would recommend chapter 1, 4, 3/2, then 5, otherwise some information in the earlier chapters might be confusing); unhelpful or busy diagrams; the beast of a chapter that is chapter 3 (overview of mass spec technology). Chapter 3 was extremely helpful, but it was also a bit of a drudge.

I still don't feel entirely confident that I would be able to analyze any mass spec data, but I have a much better idea of how it is obtained and what to look for. So overall, very helpful. I would recommend this to other people with scientific backgrounds who are interested in the nuts and bolts of proteomics.

[Thảo · Rating 5 out of 5]

Amazingly clear and well explained.