Gel Electrophoresis of Proteins: A Practical Approach

by B. David Hames (Editor)

This new edition is almost a completely new text, with eight of the ten chapters written by new authors. It presents the most reliable methods for the major procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements.

Hardcover

First published January 1, 1981

Reviews

[Elizabeth · Rating 5 out of 5]

Feel like the protocols in your lab are voodoo cobbled together from a mess of chance observations from lab members long ago, where something once worked for them so they kept doing it from then on without really understanding why it worked that one time? Ever get messy results and just had your PI shrug, say it was a "bad gel", and told you to "just repeat it", because "something was just wrong that time", and surely if you repeat it, even if you do everything the same, it will just work this time? Ever wanted to really know how a Western blot worked, but got lost in a chain of references that never actually connect to a decent review?

Then the Practical Approach series is for you. Experts actually stop and explain the voodoo: this is why you do each step, here are the proper citations for how we know what we know, and this is how to troubleshoot an ugly gel. Must-read for undergrads, first-year graduate students, and basically everyone working in a laboratory.