Methods in Cell Biology, Volume 70: Cell Biological Applications of Confocal Microscopy

by Brian Matsumoto

Cell Biological Applications of Confocal Microscopy is a comprehensive introduction to confocal microscopy for cell biologists. It contains integrated and up-to-date coverage on the various techniques and uses of this valuable research tool. Techniques are described completely and methods are made accessible and understandable, enabling the researcher to study and develop a hands-on approach to imaging. Chapters cover a variety of topics ranging from confocal microscope theory to applications for studies in cell biology. Fundamental topics include discussion on the role of coverglass thickness in 3-D imaging, and selecting a suitable pixel size. More advanced topics include the use of the microscope in ratiometric measurements with physiological dyes, the application of fast video rate microscopes, and the two-photon microscope and its advantages over traditional confocal microscopes. Chapters of interest to developmental and molecular biologists describe the properties of imaging species of animals, the Drosophilia embryo, and the single cell organism, Tetrahymena.

Key Features
* Includes detailed protocols accessible to new users
* Contains an overview of available confocal microscopes
* Details how to set up and run a "Confocal Microscope Core Facility"
* Contains over 170 figures

520 pages, Paperback

First published January 15, 1993